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Book review

Iron–Sulfur Clusters in Chemistry and Biology. Volume 2: Biochemistry, Biosynthesis and Human Diseases. Edited by Tracey Rouault. De Gruyter, 2017. Pp. xxi + 470. ISBN 978-3-11-047985-0. JP Jacquot

jacquot_acta crys 2018 (fichier PDF)

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The New Phytologist

The ectomycorrhizal basidiomycete Laccaria bicolor releases a secreted β-1, 4 endoglucanase that plays a key role in symbiosis development. F Zhang, GE Anasontzis, A Labourel, C Champion, M Haon, … The New phytologist


Abstract

In ectomycorrhiza, root ingress and colonization of the apoplast by colonizing hyphae is thought to rely mainly on the mechanical force that results from hyphal tip growth, but this could be enhanced by secretion of cell-wall-degrading enzymes, which have not yet been identified. The sole cellulose-binding module (CBM1) encoded in the genome of the ectomycorrhizal Laccaria bicolor is linked to a glycoside hydrolase family 5 (GH5) endoglucanase, LbGH5-CBM1. Here, we characterize LbGH5-CBM1 gene expression and the biochemical properties of its protein product. We also immunolocalized LbGH5-CBM1 by immunofluorescence confocal microscopy in poplar ectomycorrhiza. We show that LbGH5-CBM1 expression is substantially induced in ectomycorrhiza, and RNAi mutants with a decreased LbGH5-CBM1 expression have a lower ability to form ectomycorrhiza, suggesting a key role in symbiosis. Recombinant LbGH5-CBM1 displays its highest activity towards cellulose and galactomannans, but no activity toward L. bicolor cell walls. In situ localization of LbGH5-CBM1 in ectomycorrhiza reveals that the endoglucanase accumulates at the periphery of hyphae forming the Hartig net and the mantle. Our data suggest that the symbiosis-induced endoglucanase LbGH5-CBM1 is an enzymatic effector involved in cell wall remodeling during formation of the Hartig net and is an important determinant for successful symbiotic colonization.

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18/5/18 Séminaire de Milena Gonzalo

Milena Gonzalo
(IAM ecogeno /Dynamic)

”Understanding the molecular dialogues within forest soil microbial communities and investigating their impact on plant health and growth”


Vendredi 18 mai 2018 à 13h30
INRA (LGEF)

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4/5/18 Séminaire de Brian Looney

Brian Looney
(IAM -ecogenomic)

”Analyze of the Russulales genomes and develop an in vitro system to produce Populus-Russula ectomycorrhizae.”


Vendredi 4 mai 2018 à 13h30
INRA (LGEF)

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20/04/18 Séminaire de Veronica Basso

Veronica Basso
(IAM -ecogenomic)

”Controling hormonal balance in mutualistic interaction between trees and fungi”


Vendredi 20 avril 2018 – 13h30
INRA (LGEF)

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16/04/18 Séminaire d’Amandine Cornille

Amandine Cornille
(Génétique Quantitative et Évolution – INRA – Université Paris-Sud – CNRS – AgroParisTech)

“Adaptive genomics of plant-pest interactions: Insights from the apple-aphid system in a context of domestication”


Lundi 16 avril, 14h30
salle de conférence INRA